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Study identifier: NCT06323525 Synced from ClinicalTrials.gov · July 29, 2026
● Recruiting

TCR Reserved and Power3 (SPPL3) Gene Knock-out Allogeneic CD19-targeting CAR-T Cell Therapy in r/r B Cell Lymphoma

Condition: Non-hodgkin Lymphoma  ·  Sponsor: Chinese PLA General Hospital

PhasePhase 1/Phase 2
Planned participants30
Who can joinAll sexes, 18 Years to 70 Years
Healthy volunteersNo

About this study

The safety and efficacy of the chimeric antigen receptor (CAR)-T, a CD19-targeting, TRAC and Power3 (SPPL3) double genes deleted allogeneic CAR-T cell product, are undergoing rigorous evaluation in non-Hodgkin's lymphoma (NHL) subjects from our ATHENA trial (NCT06014073). Unexpectedly, expansion of the initial residual CD3-positive CAR T from products were measured in patients' peripheral blood (PB) without exception. Accompanying with host immune reconstitution and appearance of the detectable B cells, the CD3-positive allogenic CAR T cells exhibited a compelling amplification advantage over CD3-negative CAR T cells. The amplification of CD3-positive CAR T cell population dynamically suppressed host B cell recovery, and presumably surveilled the recurrence or progression of tumors, but did not induce typical Graft-versus-host-disease (GvHD). Additionally, a series of in vitro experiments illustrated that the HLA-mismatched fratricide between host T cells and TCR-reserved Power3 (SPPL3)-deleted allogenic CAR T cells was markedly slashed, which in combination with our observed clinical safety date supported the notion that only genomic deletion of Power3 (SPPL3) gene in allo-CAR T cells is sufficient to overcome GvHD and host T cell-mediated rejection response. In the ATHENA-2 study, our design is to preserve the expression of the TCR on T cells from healthy donors while selectively disabling the Power3 (SPPL3) gene to prepare ATHENA-2 CAR T cells. This approach harnesses the…

This description comes directly from the study's public registry record.

Talk to the study team

Weidong Han, Ph.D  ·  +86-010-55499341  ·  hanwdrsw@sina.com

Always discuss trial participation with your own doctor first.

Locations (3)

Biotherapeutic Department of Chinese PLA General HospitalBeijing, Beijing Municipality, ChinaRecruiting
Biotherapeutic Department of Chinese PLA General HospitalBeijing, ChinaRecruiting
School of Life Sciences, Peking UniversityBeijing, ChinaRecruiting

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Source record: clinicaltrials.gov/study/NCT06323525